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. 2015 Apr 15;83(5):2127–2138. doi: 10.1128/IAI.02785-14

TABLE 2.

Primer pairs used in this study

Gene and primer Sequence (5′–3′) Positionsa Product size (bp)
wbiA (BPSL2680)
    wbiA-F1 CGATTCGATGCCGCCGACGT 34 to 53 297
    wbiA-R1 CAACGCATCCGCTCGCATGC 311 to 330
    wbiA-F2 GCATGCGAGCGGATGCGTTGTGCTGCTTGGCTATCCGGCG 911 to 930 305
    wbiA-R2 TCCATCCTTGTCCGGGCCCC 1176 to 1190
    wbiA-F1 CGATTCGATGCCGCCGACGT 34 to 53 1,162
    wbiA-R2 TCCATCCTTGTCCGGGCCCC 1176 to 1190
    wbiA-Fex GGAATCTGCGTCTCCGGCTT 256 to 275 300
    wbiA-Rex ATAGGGTGTCGTGTCTCGCAG 535 to 555
    wbiA-seqA AATGGCTGCACGATGCGGTGT −104 to −124 814
    wbiA-seqB CACGGCAAGCAACACCCTGC 671 to 690
    wbiA-seqC TGCTGCGAGACACGACACCC 533 to 552 783
    wbiA-seqD GCGCAGCCGATAAAGCCAGC +57 to +76
wbiD (BPSL2677)
    wbiD-F1 CGGTGTACAGCAATGTCGTT 41 to 60 206
    wbiD-R1 CAGACGGTGCAGGTCGATTC 227 to 246
    wbiD-F2 GAATCGACCTGCACCGTCTGTCTACCGAAAGTGGCGTTCC 1409 to 1428 259
    wbiD-R2 CGGATGCCTGACAAAGAACC 1628 to 1647
    wbiD-F1 CGGTGTACAGCAATGTCGTT 41 to 60 1,607
    wbiD-R2 CGGATGCCTGACAAAGAACC 1628 to 1647
oacA (BPSL1936)
    oacA-F1 AAAGGCCGACGCATTCCGGG −162 to −144 311
    oacA-R1 AATGCCCGCTGGAGCGTGTC 130 to 149
    oacA-F2 GACACGCTCCAGCGGGCATTCCCCAAGCACCATTGGCGGT 995 to 1014 344
    oacA-R2 ATGTGGGCATGGGGAAGCGC +114 to +133
    oacA-F1 AAAGGCCGACGCATTCCGGG −162 to −144 1,480
    oacA-R2 ATGTGGGCATGGGGAAGCGC +114 to +133
wcbB (BPSL2808)
    wcbB-F1 CCGGCACTATGGCAGCCGAG 96 to 115 218
    wcbB-R1 CCATTCCGCTGTGGCTTGTATGC 291 to 313
    wcbB-F2 GCATACAAGCCACAGCGGAATGGAGCGACCTGGACGTGTTCCG 940 to 959 188
    wcbB-R2 CCACGTCGGTTCGCGGAAGT 1085 to 1104
    wcbB-F1 CCGGCACTATGGCAGCCGAG 96 to 115 1,009
    wcbB-R2 CCACGTCGGTTCGCGGAAGT 1085 to 1104
16S rRNA gene
    Univ_16S_F TGGCTCAGAACGAACGCTGGCGGC 21 to 44 336
    Univ_16S_R CCCACTGCTGCCTCCCGTAGGAGGAGT 327 to 356
    oriT-F TCCGCTGCATAACCCTGCTTC 598 to 578 236
    oriT-R CAGCCTCGCAGAGCAGGATTC 368 to 383
a

Positions correspond to the nucleotide sequences of the wbiA, wbiD, oacA, and wcbB genes of B. pseudomallei K96243 chromosome 1 (NCBI reference sequence gene identifiers 3094050, 3093124, 3091646, and 3093047 for wbiA, wbiD, oacA, and wcbB, respectively). Positions beginning with a minus sign are for genes at the 5′ untranslated region (UTR), and those beginning with a plus sign are genes at the 3′ UTR of the genes.