TABLE 2.
Primer pairs used in this study
| Gene and primer | Sequence (5′–3′) | Positionsa | Product size (bp) |
|---|---|---|---|
| wbiA (BPSL2680) | |||
| wbiA-F1 | CGATTCGATGCCGCCGACGT | 34 to 53 | 297 |
| wbiA-R1 | CAACGCATCCGCTCGCATGC | 311 to 330 | |
| wbiA-F2 | GCATGCGAGCGGATGCGTTGTGCTGCTTGGCTATCCGGCG | 911 to 930 | 305 |
| wbiA-R2 | TCCATCCTTGTCCGGGCCCC | 1176 to 1190 | |
| wbiA-F1 | CGATTCGATGCCGCCGACGT | 34 to 53 | 1,162 |
| wbiA-R2 | TCCATCCTTGTCCGGGCCCC | 1176 to 1190 | |
| wbiA-Fex | GGAATCTGCGTCTCCGGCTT | 256 to 275 | 300 |
| wbiA-Rex | ATAGGGTGTCGTGTCTCGCAG | 535 to 555 | |
| wbiA-seqA | AATGGCTGCACGATGCGGTGT | −104 to −124 | 814 |
| wbiA-seqB | CACGGCAAGCAACACCCTGC | 671 to 690 | |
| wbiA-seqC | TGCTGCGAGACACGACACCC | 533 to 552 | 783 |
| wbiA-seqD | GCGCAGCCGATAAAGCCAGC | +57 to +76 | |
| wbiD (BPSL2677) | |||
| wbiD-F1 | CGGTGTACAGCAATGTCGTT | 41 to 60 | 206 |
| wbiD-R1 | CAGACGGTGCAGGTCGATTC | 227 to 246 | |
| wbiD-F2 | GAATCGACCTGCACCGTCTGTCTACCGAAAGTGGCGTTCC | 1409 to 1428 | 259 |
| wbiD-R2 | CGGATGCCTGACAAAGAACC | 1628 to 1647 | |
| wbiD-F1 | CGGTGTACAGCAATGTCGTT | 41 to 60 | 1,607 |
| wbiD-R2 | CGGATGCCTGACAAAGAACC | 1628 to 1647 | |
| oacA (BPSL1936) | |||
| oacA-F1 | AAAGGCCGACGCATTCCGGG | −162 to −144 | 311 |
| oacA-R1 | AATGCCCGCTGGAGCGTGTC | 130 to 149 | |
| oacA-F2 | GACACGCTCCAGCGGGCATTCCCCAAGCACCATTGGCGGT | 995 to 1014 | 344 |
| oacA-R2 | ATGTGGGCATGGGGAAGCGC | +114 to +133 | |
| oacA-F1 | AAAGGCCGACGCATTCCGGG | −162 to −144 | 1,480 |
| oacA-R2 | ATGTGGGCATGGGGAAGCGC | +114 to +133 | |
| wcbB (BPSL2808) | |||
| wcbB-F1 | CCGGCACTATGGCAGCCGAG | 96 to 115 | 218 |
| wcbB-R1 | CCATTCCGCTGTGGCTTGTATGC | 291 to 313 | |
| wcbB-F2 | GCATACAAGCCACAGCGGAATGGAGCGACCTGGACGTGTTCCG | 940 to 959 | 188 |
| wcbB-R2 | CCACGTCGGTTCGCGGAAGT | 1085 to 1104 | |
| wcbB-F1 | CCGGCACTATGGCAGCCGAG | 96 to 115 | 1,009 |
| wcbB-R2 | CCACGTCGGTTCGCGGAAGT | 1085 to 1104 | |
| 16S rRNA gene | |||
| Univ_16S_F | TGGCTCAGAACGAACGCTGGCGGC | 21 to 44 | 336 |
| Univ_16S_R | CCCACTGCTGCCTCCCGTAGGAGGAGT | 327 to 356 | |
| oriT-F | TCCGCTGCATAACCCTGCTTC | 598 to 578 | 236 |
| oriT-R | CAGCCTCGCAGAGCAGGATTC | 368 to 383 |
a
Positions correspond to the nucleotide sequences of the wbiA, wbiD, oacA, and wcbB genes of B. pseudomallei K96243 chromosome 1 (NCBI reference sequence gene identifiers 3094050, 3093124, 3091646, and 3093047 for wbiA, wbiD, oacA, and wcbB, respectively). Positions beginning with a minus sign are for genes at the 5′ untranslated region (UTR), and those beginning with a plus sign are genes at the 3′ UTR of the genes.