FIG 8.

(A and C) Bar diagrams showing differential abundances of vimentin (A) and the 78-kDa glucose-regulated protein (C) at different time points post-Leishmania infection. (B and D) The expression of vimentin (B) and 78-kDa glucose-regulated protein (D) was assessed by qRT-PCR. One-way ANOVA was applied to proteomics data to analyze the fold abundance. Real-time PCR data represent means from three independent experiments. Student's t test was applied to analyze the data. RNU6A was used as an internal control. Data analysis was performed by using the 2^−ΔΔCT method. *, P < 0.05. Inf., infection. (E) Western blot analysis of PTPN6/SHP-1, GRP78, HMG-I, and histone H4 proteins across different time points in THP-1 cells infected with L. donovani. Beta-tubulin was used as a loading control. Densitometric analysis shows fold changes in expression in infected THP-1 cells with respect to the untreated control group. The data are representative of three independent experiments.