TABLE 2.
Active methyltransferase enzymes identified in VR50
| Locus tag | Coordinates | Type | R-M/orphan | Target sequencea | No. in genome | No. detectedb | % Methylatedb | Comments |
|---|---|---|---|---|---|---|---|---|
| ECVR50_3796 | 3831641–3832477 | II | Orphan | Gm6ATC | 40,144 | 39,361 | 98 | 100% amino acid sequence identity to M.EcoKDam from E .coli K-12 MG1655 |
| ECVR50_4827 to ECVR50_4829 | 4942889–4946401 | II | R-M | TGm6AN8TGCT | 775 | 755 (751) | 97.4 (96.9) | 100% amino acid sequence identity to M.EcoBI and S.EcoBI from E. coli B |
a
Underlined nucleotides reflect methylated bases on opposite DNA strand.
b
Figures for the complementary strand are in parentheses and suggest hemimethylation at 4 of 755 TGm6AN8TGCT sites.