TABLE 1.
Genotypes of Chlamydia muridarum organisms characterized in this studya
| Chromosomal variant | Affected gene | Gene length (no. of codons) | Nucleotide mutation | Protein mutation | Presence of genotype in Chlamydia muridarum clone |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| CMG0.10.1 | CMG28.54.1 | CMG0.21.3 | CMG28.12.3 | CMG0.1.1 | CMG28.38.1 | |||||
| G277313C | TC0237 | 160 | G349C | Q117E | − | + | − | + | − | + |
| −472842T | TC0412 | 366 | −84T | fs29 | + | + | − | − | − | − |
| G473020T | TC0412 | 366 | G262T | E88 stop | − | − | + | + | − | − |
| −473193T | TC0412 | 366 | −435T | fs146 | − | − | − | − | + | + |
| G797979A | TC0668 | 409 | G964A | G322R | − | + | − | + | − | − |
a
Isogenic clones were selected by pairing parental control genomes from CMG0 with TC0237(Q117E) single or TC0237(Q117E) and TC0668(G322R) double mutants from CMG28. Aside from carrying either one or both mutations in TC0237 and TC0668, isogenic pairs differ from one another by their unique TC0412 genotypes. Chromosomal variants are numbered according to the parent CMG0 consensus chromosome sequence. “fs” represents a frameshift mutation beginning at the indicated codon. “−” signifies a lack of the genotype, while “+” indicates that the clone carries the genotype.