FIG 3.

Comparison of anti-Pfs25 IgG levels among four different adjuvant groups against P. falciparum parasite lysate. Plasmodium falciparum mixed gamete/zygote stage lysate-coated ELISA plate (100 ng/well) and the pooled serum (10 mice) samples (1/500 dilutions) were used to measure anti-Pfs25 IgG levels among the four adjuvant plus alga-Pfs25-vaccinated animals AL007 (2 mg/ml alum), AL019 (0.5 mg/ml alum plus GLA), EM081 (squalene–oil-in-water emulsion), and EM082 (squalene–oil-in-water emulsion plus GLA). Titers for rPfs25 plus adjuvants are reported after subtracting signals obtained using serum from the adjuvant-alone group. Pfs25 MAb obtained from MR4 (concentration, 1 mg/ml) was used as a positive control; a sample from pooled sera of prevaccinated mice (prebleed) was used as a negative control. Statistical analysis was carried out using Student's t test between various adjuvant groups, and statistical significance is indicated by horizontal lines above the values.