FIG 4.

Western immunoblot analysis of P. falciparum mixed gamete/zygote lysate probed with anti-alga Pfs25 serum raised with four different adjuvants. Reduced (+DTT) and nonreduced (−DTT) mixed gamete/zygote stage lysates were resolved by SDS-PAGE, transferred to nitrocellulose membrane, and cut into individual strips. The blots were probed individually with postvaccinated sera from the four vaccine groups (AL007, AL019, EM081, and EM082), prebleed mouse sera (negative control), and anti-Pfs25 monoclonal antibodies (4B7) as a positive control under the same conditions to determine the comparative binding efficiency levels of anti-Pfs25 antibodies induced by different adjuvants.