FIG 5.

Pfs25-specific IgG subtypes of antibodies along with four different adjuvant groups vaccinated with alga-produced Pfs25. ELISA plates were coated with alga-produced, affinity-purified rPfs25 recombinant protein as capture antigen (100 ng/well) in bicarbonate buffer and incubated with serum samples (1/500 dilution) from different adjuvant (with rPfs25 antigen) and control (adjuvant alone) groups in duplicate wells. Groups were AL007 (2 mg/ml alum), AL019 (0.5 mg/ml alum plus GLA), EM081 (squalene–oil-in-water emulsion), and EM082 (squalene–oil-in-water emulsion plus GLA). Pooled serum samples from pre- and postimmunization sera were used for the IgG subtyping ELISA. Statistical analysis was carried out using Student's t test between various adjuvant groups, and the values mentioned for significant differences (P value < 0.05) are marked with horizontal lines above them.