FIG 8.

Adjuvant effect on transmission-blocking activity of anti-Pfs25 antibodies. Standard membrane feeding assays were carried out with pooled sera from four groups of mice vaccinated with alga-produced Pfs25 in combination with the four adjuvants (AL007, AL019, EM081, and EM082). Preimmune sera were used as negative controls. Oocyst counts reflect the successful development of P. falciparum inside the Anopheles stephensi mosquitoes. Two separate membrane feeds were done using serum from each group, and oocyst counts were pooled for statistical analysis. The table shows P. falciparum infectivity and oocyst counts in the different groups (AL007, AL019, EM081, and EM082). Statistical analysis (Mann-Whitney test and Fisher's exact test) was carried out using GraphPad Prism 5. Sig, significant.