FIG 4.

Loss of Hfq impairs the invasion of epithelial cells. (A) Confluent monolayers of eukaryotic cells were infected with C. sakazakii grown to the exponential phase and incubated for 1.5 h, followed by gentamicin treatment (100 μg/ml) for a further 1.5 h. The Caco-2 cells were lysed with 1% Triton X-100 to recover the intracellular bacteria. The data are representative of 3 independent experiments. The bars represent the means and SEM from independent experiments performed in triplicate. The asterisks indicate significant differences (***, P < 0.001). (B) Levels of OmpA and OmpX were assessed by immunoblotting in exponentially grown cells. The blots are representative of 3 independent experiments. DnaK was used as a loading control.