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. 2015 Apr 15;83(5):2089–2098. doi: 10.1128/IAI.03161-14

FIG 6.

FIG 6

Lack of Hfq results in reduced intracellular survival of C. sakazakii in macrophage-like cells. The C. sakazakii strains harboring the pACYC184 control plasmid or a complementation plasmid, pHFQ, and the wild type or hfq mutant (SK003) were incubated with RAW264.7 macrophage-like cells. (A) Intracellular bacteria of the wild type and hfq mutant (SK003) were evaluated for the number of CFU at uptake (1.5 h) and at 8, 24, 48, 72, and 96 h postinfection. (B) Intracellular bacteria of C. sakazakii strains harboring the pACYC184 control plasmid or a complementation plasmid, pHFQ, were evaluated for the number of CFU at uptake (1.5 h) and at 8 and 24 h postinfection. The bars represent means and SEM from independent experiments performed in triplicate. The data are representative of 3 independent experiments. The asterisks indicate significant differences (***, P < 0.001; **, P < 0.05).