TABLE 1.
Strain or plasmid | Genotype and/or characteristicsa | Reference or source |
---|---|---|
C. sakazakii | ||
ATCC 29544 | Wild-type strain | 15 |
ES1001 | 29544 harboring pKD46 | 15 |
SK001 | 29544 harboring pACYC184 | This study |
SK002 | 29544 harboring pBAD18 | This study |
SK003 | Δhfq | This study |
SK004 | hfq::kan harboring pACYC184 | This study |
SK005 | Δhfq harboring pACYC184 | This study |
SK006 | Δhfq harboring pBAD18 | This study |
SK007 | Δhfq harboring pHFQ | This study |
SK008 | Δhfq harboring pHFQ-A | This study |
E. coli | ||
DH5α | λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | 36 |
Plasmids | ||
pKD13 | oriR6K Ampr FRT Kanr FRT | 35 |
pKD46 | oriR101 repA101(Ts) Ampr ara BADpgam-bet-exo | 35 |
pCP20 | oripSC101(TS) Ampr Cmr cI857λ PRflp | 35 |
pACYC184 | Tetr Cmr p15A ori | 35 |
pBAD18 | Ampr araC PBAD pBR322 ori; expression vector | 37 |
pHFQ | pACYC184-hfq | This study |
pHFQ-A | pBAD18-hfq | This study |
FRT, FLP recombination target.