TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype and/or characteristicsa | Reference or source |
|---|---|---|
| C. sakazakii | ||
| ATCC 29544 | Wild-type strain | 15 |
| ES1001 | 29544 harboring pKD46 | 15 |
| SK001 | 29544 harboring pACYC184 | This study |
| SK002 | 29544 harboring pBAD18 | This study |
| SK003 | Δhfq | This study |
| SK004 | hfq::kan harboring pACYC184 | This study |
| SK005 | Δhfq harboring pACYC184 | This study |
| SK006 | Δhfq harboring pBAD18 | This study |
| SK007 | Δhfq harboring pHFQ | This study |
| SK008 | Δhfq harboring pHFQ-A | This study |
| E. coli | ||
| DH5α | λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | 36 |
| Plasmids | ||
| pKD13 | oriR6K Ampr FRT Kanr FRT | 35 |
| pKD46 | oriR101 repA101(Ts) Ampr ara BADpgam-bet-exo | 35 |
| pCP20 | oripSC101(TS) Ampr Cmr cI857λ PRflp | 35 |
| pACYC184 | Tetr Cmr p15A ori | 35 |
| pBAD18 | Ampr araC PBAD pBR322 ori; expression vector | 37 |
| pHFQ | pACYC184-hfq | This study |
| pHFQ-A | pBAD18-hfq | This study |
a
FRT, FLP recombination target.