Tracking infused stem cells using In Vivo Imaging System (IVIS) and comparison of arterial oxygen saturation and right ventricular systolic blood pressure (RVSBP) at 72 h after lung ischemia reperfusion (IR). A. Red patches (yellow arrows) around penile region indicating DiR-labelled (specific IVIS dye) adipose-derived mesenchymal stem cells (ADMSCs). B. Distribution of DiR-labelled ADMSCs around left lung field (yellow arrows) and liver (blue arrows) by post-IR 24 h. C. Persistent presence of ADMSCs (yellow arrows) over left lung field at 72 h after IR procedure. D. Arterial oxygen saturation (%) at post-IR 72 h. *vs. other bars with different symbols, p < 0.001. E. Right ventricular systolic blood pressure (RVSBP) at post-IR 72 h prior to sacrificing the animals. *vs. other bars with different symbols, p < 0.0001. All statistical analyses using one-way ANOVA, followed by Bonferroni multiple comparison post hoc test. Symbols (*, †, ‡, §) indicating significance (at 0.05 level). He-ADMSC = healthy ADMSC (i.e. cultivated in normal medium); Sd-ADMSC = serum-deprived ADMSC. (n = 6 in each group).