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. 2015 Feb 15;7(2):285–297.

Figure 7.

Figure 7

Analysis of eNOS expression after VEGF stimulation and AP-1 transfection. Endothelial cells were stimulated with or without VEGF for 24 h. Then eNOS expression at mRNA level and protein level in cells transfected with wt-27nt-miR and wt-27nt-miR+AP-1 was measured by real-time RT-PCR and Western blotting analysis, respectively. A. Quantitative real-time RT-PCR results. B. Quantitative Western blotting results. C. Representative Western blotting results. Three independent experiments were conducted and data were expressed as means ± SD. In control cells, compared to cells without VEGF treatment, ΔP < 0.01. In wt-27nt-miR group, compared to cells without VEGF treatment, ☆P < 0.01. In wt-27nt-miR+AP-1 group, compared to cells without VEGF treatment, ※P < 0.01. Compared to control cells with VEGF treatment, *P < 0.01. Compared to cells in wt-27nt-miR+AP-1 group with VEGF treatment, #P < 0.01.