Figure 6.

PO inhibited APAP and TNFα-induced activation of JNK. PO inhibited APAP and TNFα-induced activation of JNK. A. PBS and PO pretreated mice were administrated with APAP for 6h, and liver samples were then harvested for analysis of phosphorylation of JNK by western blot. (n=4~5, *p < 0.05 versus PBS+APAP). B. Normal liver cells (L02) were pretreated with PO or DMSO for 1 hour, then stimulated with 20 mg/ml TNFα for 0-45 min. Cells were then harvested and lysed for detection of JNK phosphorylation by western blot. (n=4~5, *p < 0.05 versus DMSO; for at least three different experiments).