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. 2015 Mar 14;13:88. doi: 10.1186/s12967-015-0447-7

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© Sun et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The effect of HIF2α on the self-renewal of mouse ESCs. (A) Western blot analysis of 46C mouse ESCs overexpressing empty vectors or Flag-tagged HIF2α. (B) Morphology of mouse ESCs transfected with empty or Flag-tagged HIF2α vectors and cultured in serum/LIF for five passages. Scale bar, 100 μm. (C) Cell growth assay. Relative cell proliferation was measured as a standard at Days 0, 2, 4 and 6. The original cell density was 3,000/well. Data represent the mean ± s.d. of three biological replicates. (D) Quantitative RT-PCR (qRT-PCR) analysis of Oct4, Sox2 and Nanog in mouse ESCs transfected empty- or Flag-tagged HIF2α vectors. Data represent the mean ± s.d. of three biological replicates.