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Pulse sequences utilized for the optimization of (A) 1H-13C CP, and 1H-15N CP, and (B) simultaneous CP (SIM-CP). (C) Comparison of 13C and 15N sensitivity for CP and SIM-CP for a sample of U-13C,15N microcrystalline ubiquitin. The sensitivity is almost identical for CP and SIM-CP for the aliphatic region, whereas for the carbonyl region and 15N a minimal loss of sensitivity (less than 10%) is observed using SIM-CP sequence.
