Skip to main content
. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Magn Reson. 2015 Apr;253:143–153. doi: 10.1016/j.jmr.2015.01.001

Figure 2.

Figure 2

(A) Pulse sequence to measure the bidirectional polarization transfer from N to CA and vice- versa. The CA polarization is acquired in the first acquisition, whereas 15N polarization transferred to CO and detected during the second acquisition. (B)1D spectra originating from the bidirectional polarization transfer on U-13C,15N crystalline ubiquitin using the pulse sequence shown in (A). The spectra were acquired with 32 scans and 3 ms contact periods for both NCA and NCO. The integrate intensity of carbonyl spectrum (red) is 48% of the CA spectrum (blue) due to two NC transfer periods.