Protocol of 3D MEIOSIS for simultaneous acquisition of three 3D experiments that are listed in (B), the first and second NC transfer are respectively set to NCA and NCO or vice versa. Two data sets are recorded with 15N spin lock phase set to +x and −x. Sum of the two data sets of the second acquisition gives the NCC experiment (shown in green), whereas the difference of two data sets gives NCC (shown in blue) and C(N)CC (shown in red) associated with the first and second acquisitions, respectively. (C) Simultaneous acquisition of NCACX, CA(N)COCX and NCOCX on U-13C,15-N ubiquitin using 3D MEIOIS shown in (A).