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. Author manuscript; available in PMC: 2016 Jan 5.
Published in final edited form as: Cold Spring Harb Protoc. 2015 Jan 5;2015(1):pdb.top083519. doi: 10.1101/pdb.top083519

Figure 4.

Figure 4

MPFRAP recovery curves. In both (A) and (B) MPFRAP was performed on FITC-BSA in free solution, and the data was fit to both the conventional “diffusion only” (solid line) and “diffusion-convection” (dashed line) models. (A) Solution exhibits no convective flow. Both fits yield a diffusion coefficient at 22°C of 52.9 <micro>m2/s. (B) Solution exhibits convective flow with a scaled speed of 0.8. The diffusion-only model fits the data poorly and yields a diffusion coefficient at 22°C of 160 <micro>m2/s. The diffusion-convection model fits the data well and yields a diffusion coefficient of 51.9 <micro>m2/s, in good agreement with the value determined in the absence of convective flow.