A and B, Body weight curves of male (A) and female (B) littermates fed ND or HFD ad libitum over a 30-week period starting at age 6 weeks (n = 8–11). Body weights were monitored weekly. C, Percentages of epididymal (Epi), inguinal sc (SubQ), or brown adipose tissue (BAT) depot weights to total body weight in wild-type (WT) or Nedd4+/− (Het) male mice at age 22 weeks (n = 5–7). D, Percentages of liver, gastrocnemius (Gas), or quadriceps (Qcp) weight to total body weight in WT or Het male mice at age 22 weeks (n = 5–7). E, Leptin mRNA levels in epididymal fat were measured by quantitative real-time PCR and normalized to gapdh mRNA levels (ND, n = 3; HFD, n = 5–6 with both genders). Data represent mean ± SEM. *, P < .05, **; P < .01; ***, P < .001.