Figure 3. Nedd4^+/− mice on ND have reduced insulin signaling in WAT, skeletal muscle, and liver.

A–C, ND-fed wild-type (WT) and Nedd4^+/− (Het) male mice were fasted overnight and ip injected with saline or insulin 5 U/kg body weight. Insulin-stimulated phosphorylation of IRβ and AKT in WAT (A), skeletal muscle (B), and liver (C) were measured by Western blot analysis (n = 7). Representative Western blots and quantification data were shown. Data represent mean ± SEM. *, P < .05; **, P < .01.