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. 2015 Jan 21;156(4):1283–1291. doi: 10.1210/en.2014-1909

Figure 6. HFD-fed Nedd4+/− mice display enhanced isoproterenol-stimulated lipolysis in vivo and ex vivo.

Figure 6.

A, Serum-free glycerol levels were measured in a cohort of 22-week-old wild-type (WT) and Nedd4+/− (Het) mice on ND or HFD at indicated time after ip isoproterenol, 10 mg/kg body weight (n = 5; three males and two females). B, Primary adipocytes purified from epididymal fat of WT and Het mice were incubated with or without 1 μM isoproterenol, xamoterol, or salmeterol as described in Materials and Methods. The β-antagonists ICI-118551 and CGP-20712A were preincubated for 10 minutes, and then glycerol levels in conditioned media were measured (n = 4; two males and two females). Data represent mean ± SEM. *, P < .05; **, P < .01; ***, P < .001.