Figure 7. Primary adipocytes from HFD-fed Nedd4^+/− mice are associated with increased β₂-AR protein concentrations.

A, Representative Western blots for β₂-AR expression in total tissue lysates from epididymal WAT in wild-type (WT) and Nedd4^+/− (Het) mice after 16 weeks of HFD feeding (n = 4; two males and two females). Quantification is shown in the right panel. B, Representative Western blots for β₂-AR expression in crude membrane fraction from primary adipocytes purified from WT and Het male mice after 16 weeks of HFD feeding (n = 3). Data represent mean ± SEM. *, P < .05.