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The effect of HE dsRNA on induced CPEs in infected ASK cells. (A) Uninfected ASK cells at 11 days post-infection (B) ASK cells infected with HPR35 ISAV without treatment and (C) with the addition of E. coli carrying dsRNA against HE. (D) Infected ASK cells and treatment with E. coli HT1115 carrying empty vector pL4440 was included as a control. The CPEs observed in ASK cells infected as described were vacuolated cells and monolayer detachment. The scale bar represents 20 μm.
