Figure 10. Kinetics of expression in the course of neural regeneration.

(A) Hm-MyD88 and (B) Hm-SARM gene and protein expressions were assessed by qRT-PCR and western blot in isolated nerve cords cultured ex vivo for up to 8 days under sterile conditions. Samples were collected at 0 h (T0), 6 h, 1 day, 3 days and 8 days. qRT-PCR results are presented as graphics. Graphics represent the best results of two independent experiments that displayed similar variations; p-values from Student's T-tests were calculated between the different conditions, based on experimental measurements performed in triplicate (*p < 0.05). For western blot experiments, membranes were incubated with anti-Hm-MyD88 and anti-SARM. To assess that an equal amount of proteins was loaded on gels, membranes were stripped and reprobed with anti-Actin.