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. 2015 Apr 16;5:9624. doi: 10.1038/srep09624

Figure 5. Hm-MyD88 is expressed in the central nervous system of the leech.

Figure 5

(A) Western blot analysis of leech CNS extracts with anti-Hm-MyD88. To assess the specificity of anti-Hm-MyD88, two controls were performed: An incubation with Hm-MyD88 antibody pre-incubated with the blocking peptide and a reaction with the pre-immune serum. PTM: Post translational modifications. (B) Detection of Hm-MyD88 transcript by RT-PCR in leech purified neurons and microglial cells. (C) Cellular localization of Hm-MyD88 in neurons and microglia was determined by immunofluorescence and confocal microscopy analyses. No immunolabeling was observed when incubated isolated neurons with the pre-immune serum.