Figure 2.

Antibiotic treatment reduces rotavirus (RV)–induced diarrhea in neonatal mice. C57BL6 pregnant dams were treated with ampicillin and neomycin in drinking water ad libitum 1 week before giving birth, and treatment was continued until offspring were weaned. Offspring were treated with 100 µg of neomycin and 200 µg of ampicillin in 100 µL of water by oral gavage 1 day before and 1 day after inoculation. Six-day-old mice were inoculated with 2 50% diarrhea doses of mouse RV and were visually observed for diarrhea daily. A, Typical RV diarrhea in neonates. B, Daily rates of observable diarrhea on days 0–9 after inoculation. C, Incidence of diarrhea, represented as both a fraction and percentage of total mice inoculated. D, Of the mice that showed evidence of diarrhea, the numbers of days each mouse had diarrhea was averaged. *P < .05.