Figure 7.

Increasing basal immune activation impairs rotavirus (RV)–induced antibody generation. C57BL6 mice were treated with dextran sodium sulfate (DSS) as described in Materials and Methods and inoculated with RV. A, Fecal lipocalin-2 expression was analyzed with enzyme-linked immunosorbent assay. B, Feces specimens were collected daily, and RV antigen was assayed. C, E, and G, Serum and fecal supernatant was obtained, and serum RV immunoglobulin G (IgG; C), serum RV immunoglobulin A (IgA; E), and fecal RV IgA (G) production, as measured by RV immune reactivity at a single dilution of serum or fecal supernatant, was probed up to 9 weeks after inoculation. D, F, and H, Serum RV IgG (D), serum RV IgA (F), and fecal RV IgA (H) titers, as measured by the sample dilution at which OD₄₅₀ equaled 0.2 over blank, at 9 weeks after inoculation. *P < .05.