Table 2.
Oligonucleotide pairs used for determining gene expression of Lactobacillus bulgaricus
| Protein | Gene | Primer sequence (5' to 3') | Product (bp) | T m (°C) |
| 16S ribosomal RNA | 16S rRNA | Sense: ATCGGAAACTGTCATTCTTG; Antisense: CTAATCCTGTTCGCTACCC | 157 | 51.6 |
| Oligopeptide transporter | OppD | Sense: CCAGTTTGAACCCTTTGA; Antisense: CTTAGGCATCCCTACTTGAT | 128 | 51.3 |
| Cell-envelope proteinase B | PrtB | Sense: GTGGCAATTCAAATCTAATC; Antisense: GCTGTAATCATCACCCTCAT | 107 | 47.9 |
| Aminopeptidase C | PepC | Sense: CAAGCCGACTAAGTGGAAG; Antisense: AACAGCCTGGTAAACGAA | 109 | 50.1 |
| Endopeptidase F | PepF | Sense: TATCGCAGTGAGGTTCC; Antisense: TTCTTCTGGTTGCTGGTGT | 254 | 53.8 |
| Proline peptidase Q | PepQ | Sense: ACTGATAAGATGCGGGAAAT; Antisense: GCAAAGCCTGGGATGTAG | 275 | 54.8 |
| Proline peptidase X | PepX | Sense: TATTCAACGGCAAGTCCC; Antisense: TCCCGCCAAAGTAAGGTG | 195 | 54.9 |
| Tripeptidase T | PepT | Sense: TACGGACGGGTCACAACT; Antisense: GTCAACAGCCTTCCACATT | 121 | 51.7 |
T m: melting temperature