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. 2015 Mar 25;5:9482. doi: 10.1038/srep09482

Figure 4. Tumor-induced ADPR production by intracellular CD38 is responsible for sustained Ca2+ signals in NK cells.

Figure 4

(a and b) Tumor cells induce ADPR production but cADPR in Cd38+/+ NK cells. Levels of ADPR and cADPR were determined after treatment of NK cells with 30 μg PME prepared from B16F10 tumor cells for 40 s. Data are mean ± SEM of three independent experiments. *P < 0.001; #P < 0.05. ns, not significant. (c–e) Cibacron blue 3GA (CB) inhibits of tumor-stimulated ADPR induction (c), sustained Ca2+ signal (d), cytotoxicity (e). CB (100 μM), ara-2′-F-NAD (200 nM), bafilomycin A1 (200 nM) or GPN (50 μM) was preincubated for 30 min. Data shown in c–e are representative of three independent experiments. *P < 0.001 vs basal; #P < 0.05.