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. 2015 Apr 9;5:9357. doi: 10.1038/srep09357

Figure 2. Knocking-down ERH impaired DNA-damage-repair ability.

Figure 2

(A) Expression of ERH mRNA and protein in HepG2 cells upon ERH siRNA transfection and in ERH open reading frame stably expressing HepG2 cells upon siRNA ERH 5 transfection (siERH 5 rescue). (B) Representative images of comet assay in HepG2 cells upon ERH knocking-down and ERH rescue, followed by one episode of UV 200 J/m2 irradiation. Cells were collected at 0, 1 and 24 hours after irradiation. (White arrows indicated damaged DNA) (C) Quantification of comet assay in figure 2B.