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. 2015 Apr 9;5:9357. doi: 10.1038/srep09357

Figure 3. ERH modulated DNA damage response in HCC cells.

Figure 3

(A) Expression of ERH mRNA and protein in HepG2 cells upon ERH siRNA transfection and in ERH open reading frame stably expressing HepG2 cells upon siRNA ERH 5 transfection (siERH 5 rescue). (B) ERH protein expression didn't change after UV 100 J/m2 irradiation or hydroxyurea 20 mM treatment. (C)Protein expression of total and phosphorylated ATR protein upon ERH knocking-down in HepG2 cells after UV 100 J/m2 irradiation of various durations. (D) Protein expression of total and phosphorylated Chk1 protein and gamma-H2AX expression upon ERH knocking-down after UV irradiation. (E) Protein expression of total and phosphorylated ATR protein upon ERH knocking-down in HepG2 cell upon hydroxyurea 20 mM treatment of various durations. (F) Protein expression of total and phosphorylated Chk1 protein and rH2Ax expression upon ERH knocking-down after hydroxyurea treatment. (G) ATR pre-mRNA expression at the exon–intron junctions detected by qPCR with exon-spanning and splice junction primers. The schematic indicates the location of PCR primers (EE, exon–exon PCR; EI, exon–intron PCR; IE, intron–exon PCR).