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. Author manuscript; available in PMC: 2015 Apr 16.
Published in final edited form as: J Cancer Ther. 2012 Oct;3(5):504–510. doi: 10.4236/jct.2012.35065

Figure 1.

Figure 1

Taxol-induced apoptosis and GM3 synthase expression in SKOV3 cells. (a) Cells were treated with 100 nM Taxol for 48 h, harvested, and apoptosis was determined by FACS analysis as described in Materials and Methods. Compensation was executed for each experiment using untreated cells and cells stained only with annexin V or propidium iodide, respectively. Error bars show standard deviation from triplicate measurements (*P < 0.05). (b) Total RNA from SKOV3 cells was extracted and the levels of GM3 synthase mRNA were measured by RT-PCR. β-actin was used as an internal control in the RT-PCR reactions. (c) SKOV3 and SKOV3/GS cells were treated with 100 nM of Taxol for 48 h and apoptotic cell death was determined by DAPI staining as described in Materials and Methods (c1 and c3 are control untreated SKOV3 and SKOV3/GS, respectively; c2 and c4 SKOV3 and SKOV3/GS cells treated with 100 nM Taxol, respectively). (d) Apoptotic cells were identified by condensation and fragmentation of nuclei. A minimum of 300 cells were counted for each treatment, and the percentage of apoptotic cells was calculated. Error bars show S.D. from triplicate measurements.