Figure 3.
GM3 synthase increases proliferation and decreases Taxol cytotoxicity in SKOV3/GS cells. (a) SKOV3 and SKOV3/GS cells were grown for 48 h and the number of cells was determined as described in Materials and Methods. (b) SKOV3 and SKOV3/GS cells were treated with 1 – 100 nM Taxol for 48 h and the numbers of cells which survived was determined by methylene blue cytotoxicity assay as described in Materials and Methods. (c) SKOV3 cells untreated and SKOV3 cells treated with 5 or 10 nM Taxol for 48 h; SKOV3/GS untreated cells and SKOV3/GS cells treated with 5 or 10 nM Taxol for 48 h as described in the Materials and Methods. Colonies were counted 7 days later. The values are representative of triplicate counts and error bars show standard deviations.
