Table 2.
Proposed Secondary Markersa of Young Healthy NP and NP Progenitor Cells and Their Relevance to NP Cell Physiology
| Identified NP phenotypic marker | Relevance to NP physiology | Species | Method |
|---|---|---|---|
| Integrins α3, α6, β4 | Cell-matrix adhesion | Human, porcine55,56 | Flow cytometry, IHC |
| Microarray and proteomics studies | |||
| Annexin A3 | Unknown | Rat46 | Microarray |
| Glypican 3 | Rat46 | Microarray | |
| α2-macroglobulin | Human, dog47,64 | Microarray | |
| Desmocollin-2 | Dog64 | Microarray, IHC | |
| CD56 | Dog, mouse64,77 | Microarray, flow cytometry, IHC | |
| SNAP25 | Bovine, human39 | Microarray, qPCR | |
| CDH2 | Bovine, human39 | Microarray, qPCR | |
| BASP1 | Bovine, rat39,83 | Microarray | |
| SOSTDC1 | Bovine39 | Microarray | |
| PAX1 | Human67,71 | Microarray, qPCR | |
| FOXF1 | Human67,71 | Microarray, qPCR | |
| Hemoglobin β-chain | Human67 | Microarray | |
| Ovostatin | Human67 | Microarray | |
| Neurochondrin | Rat79 | Microarray, qPCR, IHC | |
| Neuropilin-1 | Rat79 | Microarray, IHC | |
| CD155 | Rat79 | Microarray | |
| CD221 | Rat79 | Microarray, IHC | |
| Lubricin (PRG4) | Human73,74 | IHC, LC-MS/MS | |
| Progenitor markers | |||
| Tie2 | Receptor for angiopoietin-1; drives proliferation of progenitor cells | Human, mouse77 | IF, flow cytometry |
| GD2 | Unknown | Human, mouse77 | IF, flow cytometry |
a
Secondary markers were identified in the literature but less well validated for young healthy NP cells than primary markers.