Fig 3. Effect of metformin on phosphorylation of Akt, mTOR, p70S6, and AMPK-α in the liver of experimental mice.

(A) Total protein was extracted from the livers of experimental mice and expression of Akt, p-AKT, mTOR, p-mTOR, p70S6, p-p70S6, AMPK-α, and p-AMPK-α was examined by western blot. Repeat western blots gave similar results. (B) Blot intensities were quantified using densitometry. Columns and lines indicate means and SD of triplicate assays. * P < 0.05, ** P < 0.01, and *** P < 0.001.