Table 1. Single-Turnover DNA Cleavage Rate Constants (k_obs) using 1 μM SgrAI dimer.

32P-labeled Reporter DNA (1 nM)	Added Unlabeled Activator DNA	kobs a (min-1) a	Percent Cleaved a , b	Relative Acceleration c
18–1	0 nM PC d	0.094±0.015	-	-
	10 nM PC d	0.18±0.06	-	2
	100 nM PC d	0.30±0.03	-	3
	250 nM PC	12.6±0.8	74±4	130
	500 nM PC	27±7	90±10	300
	1000 nM PC d	22±7	-	230
	1000 nM 22–1 e	1.1±0.4		12
	1000 nM 22-1-3’S	1.47±0.12	-	16
40–1	0 nM PC	0.236±0.006	68±1	-
	10 nM PC	0.37±0.01	71±1	1.5
	100 nM PC	4.4±0.4	69±2	20
	250 nM PC	11.0±0.3	71.6±0.4	50
	500 nM PC	18.0±0.3	75.8±0.2	80
	1 μM PC	16.3±1.7	76.3±0.4	70
	2 μM PC	23±2	73.0±0.8	98
40–1	1 nM 40–1	0.28±0.02	63±2	-
	1 μM 40–1	12±1	29±3	40
40-2A	0 nM PC	0.013±0.004	17.7±0.5	-
	10 nM PC	0.018±0.004	70±7	1
	100 nM PC	0.039±0.005	63±2	3
	250 nM PC	0.82±0.09	28±2	60
	500 nM PC	6.1±0.6	53±1	500
	1 μM PC	9±3	53±7	700
	2 μM PC	10±3	38±3	800
40-2A	1 nM 40-2A	0.004±0.003	34±12	-
	1 μM 40-2A	0.25±0.06	78±5	20
40-2B	100 nM PC	0.0221±0.0009	54±3	2 f
	250 nM PC	1.23±0.07	20±4	100 f
	500 nM PC	2.2±0.4	29±2	170 f
	1 μM PC	8.1±1.4	33±2	600 f
	2 μM PC	11.8±1.4	45±2	900 f
40-2B-Top	0 nM PC	0.0129±0.0004	69.7±0.2	-
	1 μM PC	9.3±1.6	55.2±0.3	700
40-2B- Bottom	0 nM PC	0.0023±0.0009	46±17	-
	1 μM PC	17±4	50±1	7000
40-NC-TA	0 nM PC	0.024±0.004	11±1	-
	1 μM PC	0.052±0.006	5.2±0.6	2

^aValues presented at the average of three independent trials ± the standard deviation.

^bPercent Cleaved is the percentage of DNA cleaved by SgrAI in the assay by the given rate constant (k_obs). In cases where less than 100% is cleaved by the given rate constant, the remainder is cleaved by a nonaccelerated rate constant.

^cRelative Acceleration is the ratio of k_obs to that in the absence of added unlabeled DNA.

^dFrom Park, et al., 2010[8].

^e From Park, et al., 2010[10].

^fRelative to cleavage of 40-2B-Top with no PC DNA.