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. 2015 Apr 16;10(4):e0123938. doi: 10.1371/journal.pone.0123938

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© 2015 Leskela et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — Levels of mRNA of Gal-1, Gal-9, Tim-3 and the indicated Th1/Th2 cytokines were determined by qRT-PCR analysis in thyroid samples from 40 AITD patients and 22 patients with goiter. A-C) mRNA levels of Gal-1, Gal-9 and its ligand Tim-3 in thyroid tissue from patients with goiter, HT and GD. D) Correlation analysis between Tim-3 and Gal-9 mRNA levels in thyroid tissue samples from patients with GD. E-H) mRNA levels of the indicated Th1 and Th2 cytokines in thyroid tissue from patients with goiter, HT and GD. I) Correlation analysis between mRNA levels of Gal-9 and the indicated cytokines in thyroid tissue samples from patients with GD. mRNA levels were normalized using HPRT and β-actin as internal controls. Differences among groups were analysed by the ANOVA and Bonferroni tests, *p<0.05, **p<0.01, ***p<0.001. Correlation analysis was performed by Pearson or Spearman tests.