Levels of mRNA of Gal-1, Gal-9, Tim-3 and the indicated Th1/Th2 cytokines were determined by qRT-PCR analysis in thyroid samples from 40 AITD patients and 22 patients with goiter. A-C) mRNA levels of Gal-1, Gal-9 and its ligand Tim-3 in thyroid tissue from patients with goiter, HT and GD. D) Correlation analysis between Tim-3 and Gal-9 mRNA levels in thyroid tissue samples from patients with GD. E-H) mRNA levels of the indicated Th1 and Th2 cytokines in thyroid tissue from patients with goiter, HT and GD. I) Correlation analysis between mRNA levels of Gal-9 and the indicated cytokines in thyroid tissue samples from patients with GD. mRNA levels were normalized using HPRT and β-actin as internal controls. Differences among groups were analysed by the ANOVA and Bonferroni tests, *p<0.05, **p<0.01, ***p<0.001. Correlation analysis was performed by Pearson or Spearman tests.