Fig 1. Sampling design and formation of the groups.

(A) Sampling design for the study and (B) Scheme of the experimental groups. The letters M and F represent the male and female offspring, respectively (in A). Circles represent the female sex, and squares represent the male sex (in B). Abbreviations: Standard-chow group (SC); high-fat group (HF); father (Fa); mother (Mo). Groups—Father that received standard chow (SC-Fa); Father that received high-fat diet (HF-Fa); mother that received standard chow (SC-Mo); mother that received high-fat diet (HF-Mo); offspring of SC mother and SC father (SC-Mo/SC-Fa); offspring of SC mother and HF father (SC-Mo/HF-Fa); offspring of HF mother and SC father (HF-Mo/SC-Fa); offspring of HF mother and HF father (HF-Mo/HF-Fa).