Table 2. RT-qPCR primers and respective sequences.
Name | 5–3’ | Primers |
---|---|---|
CPT-1 | FW | AAGGAATGCAGGTCCACATC |
CPT-1 | RV | CCAGGCTACAGTGGGACATT |
PPAR-α | FW | TCGAGGAAGGCACTACACCT |
PPAR-α | RV | TCTTCCCAAAGCTCCTTCAA |
SREBP-1c | FW | TCTGCCTTGATGAAGTGTGG |
SREBP-1c | RV | AGCAGCCCCTAGAACAAACA |
FAS | FW | CATCCAGATAGGCCTCATAGAC |
FAS | RV | CTCCATGAAGTAGGAGTGGAAG |
PEPCK | FW | AGCTGCATAATGGTCTGG |
PEPCK | RV | GAACCTGGCGTTGAATGC |
G6Pase | FW | AACGCCTTCTATGTCCTCTTTC |
G6Pase | RV | GTTGCTGTAGTAGTCGGTGTCC |
β-actin | FW | TGAGACCTTCAACACCCCAGCCA |
β-actin | RV | CGTAGATGGGCACAGTGTGGGTG |
Abbreviations: carnitine palmitoyltransferase I (CPT-1); peroxisome proliferator activator receptor (PPAR)-alpha, sterol regulatory element binding protein (SREBP)-1c; fatty acid synthase (FAS); phosphoenolpyruvate carboxykinase (PEPCK); glucose-6-phosphatase (G6Pase).