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. 2015 Apr 16;9(4):e0003714. doi: 10.1371/journal.pntd.0003714

Fig 3. Extravascular trypanosomes appear in the meninges.

Fig 3

A. GFP trypanosomes in the meninges at 32 dpi. Blue: collagen. Faint magenta: blood marker (Qdots). B. At the level of vertical penetrating vessels and parenchymal capillaries, intravascular trypanosomes were detected (arrow), but extravascular trypanosomes were not. 16 dpi. C. A trypanosome labeled by intravenous injection of furamidine. In this case, the nucleus gave red fluorescence (arrow) and the cytoplasm gave blue fluorescence. D. Trypanosomes (identifiable by their rapid movement as in S5 Video) giving red fluorescence after labeling with i.v. furamidine. Furamidine also produced blue fluorescence from endogenous nuclei. Single plane, 25 dpi, CD-1 mouse. E. Numbers of trypanosomes in the meninges as a function of dpi (green triangles). Their appearance tends to be later than the increase in T cells (red lozenges). In 8 mice, T cell number had increased but no trypanosomes were observed in the meninges. F. The number of trypanosomes in the dura shows no dependence on paristemia. G. Numbers of trypanosomes in the dura 20–40 dpi in C57BL/6 mice and CD1 mice (from [20]).