Fig. 2.
A–E Properties of nuclear pore channel (NPC) gating in Dunning G prostate cancer cells. A single experiment, demonstrating the statistical analysis used throughout the patch-clamp investigations. Each individual record of patch current, Ip, was inspected and single-channel conductance (γ) calculated. The statistical properties were calculated from the eight-record ensemble. Open–close and close–open transitions were analyzed by direct inspection of each current trace of the ensembles and by analysis of their current histograms A–D. From these, open and close probabilities were evaluated. To evaluate the linearity, and thus the voltage dependence, of γ alternating positive and negative pulses were delivered from a holding potential of 0 mV. A Graphs corresponding to pulses from 0 to −10 mV (pipette potential). B–D Results for pulses from 0 to +10, −20, and +20 mV, respectively. E Current–voltage plot obtained from 35 measurements of discrete current transitions, Δi