Fig. 3.
A–C Ca2+ and ATP treatment causes transient plugging of nuclear pores. A Record ensembles of patch conductance, Γp(t)=Ip(t)/V, obtained at various times of bath application of 1 µM [Ca2+] plus the Ca+ATP-regenerating system (see Materials and methods). B Relative values of the mean of the average traces, , corresponding to the ensembles in A. C Cumulative histograms for the ensembles in A. The calculated single-channel conductance is given for each histogram. Ensembles were generated by applying voltage pulses to the pipette electrode from a holding potential of 0 to a test potential of −10 mV