Figure 1.

(A) Morphological assessment of eSF after 14-days treatment with 10 nM estradiol + 1 µM progesterone (E₂P₄). eSF from controls (dCtrl) and 8/12 women with PCOS (dPCOS) decidualized but eSF from 4/12 women with PCOS did not (ndPCOS). (B) PGR and insulin-like growth factor binding protein 1 (IGFBP-1) mRNA expression after vehicle (V) or steroid hormone (10 nM E₂ or E₂P₄) treatment. PGR expression was comparable between the study groups whereas ndPCOS presented with decreased IGFBP-1 mRNA expression. (C) IGFBP-1 response was also decreased at the protein level (ELISA) in ndPCOS compared with other study groups. Only cells treated with E₂P₄ are shown. No IGFBP-1 was detected after incubation with V or E₂.