Figure 2.

Multiplex protein analysis (Luminex) of different cytokines (pg/10⁵ cells) after 14-days treatment with vehicle (V) or 10 nM estradiol (E₂), or 10 nM E₂ plus 1 µM progesterone (P₄). (A) Interleukin 6 (IL-6). (B) Interleukin 8 (IL-8). (C) Monocyte chemoattractant protein-1 (MCP-1). (D) Regulated on Activation, Normal T Cell Expressed (RANTES). (E) Granulocyte-macrophage colony-stimulating factor (GM-CSF). DCtrl, eSF from controls: dPCOS eSF from women with PCOS (8/12) that exhibit decidualization; ndPCOS, eSF from women with PCOS (4/12) that did not decidualize. The significant differences (P < 0.05) are marked as follows: − (line) between the different treatment groups within the study group (dCtrl or dPCOS or ndPCOS) and * (asterisk) compared with dCtrl and † (cross) compared with dPCOS in the same treatment group between different study groups.