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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Neurochem. 2015 Jan 28;133(4):532–543. doi: 10.1111/jnc.12988

Fig. 2.

Fig. 2

Characterization of 5-HT1A and 2A receptors in developing OLs by Western blot and intracellular Ca2+ assay. A: Both 1A and 2A receptors at predicted molecular weight were detected by Western blot in OL developing OLs. B: Intracellular Ca2+ increased in immature OLs upon 5-HT application (1 μM). Date represent a net increase of intracellular Ca2+ levels over 10 min period. N=6 in each group. *p<0.05 vs the control. C. Representative graphs show dynamic changes of intracellular in the control (medium only), 5-HT, and AMPA (used as a control) treated cells in a single well.