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. 2015 Mar 31;38(4):304–311. doi: 10.14348/molcells.2015.2122

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Fig. 6. — Analysis of apoptosis in cultured granulosa cells transfected with let-7 mimics. Granulosa cells transfected with negative control (NC) (A), let-7a/b/c/i mimics (B) or let-7g mimic (C) were stained with Annexin V and PI. The apoptosis level of each group was analyzed by flow cytometry. Analysis of different apoptosis rates in NC, let-7a/b/c/i co-transfection group and let-7g transfection group (D). Data are presented as mean ± SE. A one-way ANOVA test was used for multiple comparisons. P < 0.05 is shown as *. Morphological changes in granulosa cells transfected with NC, let-7a/b/c/i and let-7g mimics (from left to right) were stained with Hoechst33342 and images were obtained under a fluorescence microscope (E) (bar = 20 μm).