Fig. 3.

Apoptotic activation of caspase-8 is highly dependent on Eph receptor subtype. (A–F) HEK293 cells were transiently transfected with the indicated constructs as described in Fig. 3. Note that EphA8 does not promote apoptotic cell death even in the presence of caspase-8. (G) Cleaved caspase-3 stained apoptotic cells were counted as described in Fig. 3E but are presented as a percentage of total GFP⁺ cells. Caspase-8 was subcloned into a bicistronic pIRES-GFP vector. Data represent ± standard errors. *P < 0.001; single-factor analysis of variance.